A seven-residue deletion in prp leads to generation of a spontaneous prion formed from c-terminal c1 fragment of prp

HIGHLIGHTS

• who: Carola Munoz-Montesino from the Plasmid constructs Sheep Prnp ORF encoding PrP allotype VRQ (Val136, Arg154, and Gln171) was cloned into pTRE plasmid of the pTeT-on expression system (Clontech) (27)Deletions of residues HTVTTTT (positions, ) and, terminal deletions, and, to generate WT and mutant versions of , and , were performed by site-directed mutagenesis (QuikChange II mutagenesis kit have published the article: A seven-residue deletion in PrP leads to generation of a spontaneous prion formed from C-terminal C1 fragment of PrP, in the Journal: (JOURNAL) of July/27,/2020
• what: This work . . .
  

   

  

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