A simple and efficient method for the substrate identification of amino acid decarboxylases

HIGHLIGHTS

  • who: Mingyu Fang et al. from the Shanghai Key Laboratory of Metabolic Remodeling and Health, Institute of Metabolism and Integrative Biology, Fudan University, Shanghai, China have published the Article: A Simple and Efficient Method for the Substrate Identification of Amino Acid Decarboxylases, in the Journal: (JOURNAL)
  • what: The authors used GAD65 and AADC to demonstrate the method4for the subExpression and purification of theacid amino_acid decarboxylase.
  • how: The authors chose and as models to validate the method . and were expressed in HEK 293T cells and purified through immunoprecipitation. For the Substrate Identification of Aminoour . . .

     

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