A simple and rapid technique of template preparation for pcr

HIGHLIGHTS

  • who: ufeffJianpingufeff ufeffXuufeff and ufeffZhiminufeff ufeffLiufeff from the Technology, United States University, Hamilton, ON, Canada have published the research: A simple and rapid technique of template preparation for PCR, in the Journal: (JOURNAL)
  • what: The authors develop an alternative and highly efficient protocol to eliminates these problems.
  • how: After KOH was identified as the best among the three alkaline agents for PCR template preparation (please see results below) the authors conducted a concentration optimization experiment. Genes and primers used in this study To assess the effectiveness of prepared DNA as templates for PCR . . .

     

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