HIGHLIGHTS
SUMMARY
For a given locus, the CB-RNA reflects the sum of elongating RNA (RNAe: RNAs being transcribed at the locus) and full-length polyadenylated RNA (RNAf: RNAs have been fully transcribed and polyadenylated but not yet released from chromatin). The data showed that polyadenylated RNA likely represents the intermediate of on-chromatin RNA processing and accounts for only a small fraction of CB-RNAs. Assessment of nascent RNA enrichment in the isolated CB‑RNAs by qPCR CB-RNAs isolation captures all the chromatin-associated RNA species, including the nascent transcripts containing introns that are . . .
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