A single active-site mutagenesis confers enhanced activity and/or changed product distribution to a pentalenene synthase from streptomyces sp. pska01

HIGHLIGHTS

  • who: Hongshuang Liu et al. from the State Key Laboratory of Bio-Based Material and Green Papermaking, School of, Qilu University of Technology, Shandong Academy of Sciences, Jinan, China have published the paper: A Single Active-Site Mutagenesis Confers Enhanced Activity and/or Changed Product Distribution to a Pentalenene Synthase from Streptomyces sp. PSKA01, in the Journal: Bioengineering 2023, 392 of /2023/
  • what: The authors constructed pentalenene synthetic pathway in E. coli BL21(DE3) and generated PentS variants with random mutations to construct mutant library. mutant PentS-13 with varied product diversity was obtained through . . .

     

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