A single-plasmid approach for genome editing coupled with long-term lineage analysis in chick embryos

HIGHLIGHTS

• who: Retroviruses et al. from the (UNIVERSITY) have published the article: A single-plasmid approach for genome editing coupled with long-term lineage analysis in chick embryos, in the Journal: (JOURNAL) of 23/02/2021
• what: Using transfection- and electroporation-based approaches the authors show that this construct can be used to perturb gene function in early embryos as well as human cell lines. The authors demonstrate the application of the newly engineered constructs and viruses by perturbing β-catenin in vitro and Sox10 Pax6 and Pax7 in the retina and tube and segmental plate in . . .
  

   

  

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