A tailored strategy to crosslink the aspartate transcarbamoylase domain of the multienzymatic protein cad

HIGHLIGHTS

  • who: Francisco del Cau00f1o-Ochoa and collaborators from the Instituto de Biomedicina de Valencia (IBV), CSIC, Jaime Roig, Valencia, Spain have published the research: A Tailored Strategy to Crosslink the Aspartate Transcarbamoylase Domain of the Multienzymatic Protein CAD, in the Journal: Molecules 2023, 28, 660. of 14/Nov/2022
  • what: The authors propose that this cysteine crosslinking is suitable strategy to strengthen the contacts between subunits in the CAD particle and facilitate its structural characterization. For this study, the authors engineered a construct encoding only the ctATC domain Purified aa ctATC2Cys formed crystals less thanbinding . . .

     

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