A20 targets pfkl and glycolysis to inhibit the progression of hepatocellular carcinoma

HIGHLIGHTS

• who: Yilu Feng from the Huh, cells were purchased from American Type Culture Collection and , cells were provided by the Cancer Research Institute of Central South University (Changsha, China)All cell lines were used within, passages of thawing from the original stocks and were tested every , months for mycoplasma contamination. The cell lines were maintained for no more than , passages between experiments. We used DMEM/ High Glucose medium (Gibco, CT BT) supplemented with, % fetal bovine serum (Biological Industries, C), % penicillin and streptomycin (HyClone, SV30010) to culture Huh, and , cells as previously described20. All cells were maintained in . . .
  

   

  

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