Absolute measurement of cellular activities using photochromic single-fluorophore biosensors and intermittent quantification

HIGHLIGHTS

• who: Franziska Bierbuesse from the Department University of have published the article: Absolute measurement of cellular activities using photochromic single-fluorophore biosensors and intermittent quantification, in the Journal: NATURE COMMUNICATIONS NATURE COMMUNICATIONS of 27/08/2020
• how: Source data are provided with this paper.

SUMMARY

The authors next characterized their spectroscopic properties in_vitro (Fig 1, Table 1 and Supplementary Figs S4-S9). The authors opted to use the in_vitro calibration as it is obtained in welldefined conditions for which the Ca2+ concentration is unambiguously known. The authors used this to visually . . .

 



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