HIGHLIGHTS
- who: Yanbo Wang from the Centroid of FISH spot Relative distance have published the research: Achieving single nucleotide sensitivity in direct hybridization genome imaging, in the Journal: (JOURNAL) of 19/02/2022
- what: The authors develop single guide (sg) version of GOLDFISH (genome oligopaint via local denaturation FISH)14 to address the technical gap in direct hybridization SNV detection. The authors show that DNA cleavage efficiency of a specificity-enhancing Cas9 variant in conjunction with a guanine-extended gRNA is sensitive to single base_pair differences in_vitro and in fixed cells. The authors demonstrate allelespecific visualization . . .
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