Acta v29_n2_art05_pg207a212_id_3755.indd

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  • who: Usuario from the Botanical material Ripe fruits of Ricinus communis L(Euphorbiaceae) occurring in areas of the Universidade Federal de Santa Maria (UFSM) (Santa Maria, Rio Grande do Sul, Brazil) were collected. Fixation After collection, the fruits were dissected, the seeds were removed, and the endosperm and embryo were separated. The entire dissection process was performed on a Petri dish containing, ., M sodium phosphate buffer (pH, .2). The embryos were transversally sectioned in the Petri dish in slices of approximately , mm thickness. After dissection, the embryo slices were placed in microcentrifuge tubes containing the fixative solution . . .

     

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