HIGHLIGHTS
SUMMARY
Several studies have used SERCA-positive membrane subpopulations to either validate the identity of a putative junctional SR protein, or non-junctional SR protein. To the enrichment of junctional SR proteins, MedSR membranes were also enriched in peroxisomal proteins, rab proteins, and caveolar proteins (Fig 7), but the three types of protein-containing vesicles varied in their membrane enrichments. The authors used the technique of Ca oxalate loading of cardiac SR membrane vesicles to produce two SR membrane subpopulations (MedSR and HighSR), thereby enriching patches of cardiac ER/SR membrane that contain combinations of . . .
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