Activation of cdc42 is necessary for sustained oscillations of ca and pip stimulated by antigen in rbl mast cells

HIGHLIGHTS

• who: G et al. from the The genetically encoded calcium indicator, GCaMP3, previously described (Tian et_al, ), was purchased from Addgene (plasmid no22692). DNA plasmids pcDNA3.0, Cdc, G V, Cdc, G V/QQ, Cdc, T N, Dock7, and Cool, were generously provided by Dr Richard Cerione (Cornell University, Ithaca, NY). Super-ecliptic pHluorin, as a fusion with transferrin receptor (human) in the vector j, (Merrifield et al., ), was obtained from Dr P. De Camilli (Yale University, New Haven, CT). To create the VAMP8-pHluorin-fusion construct, the transferrin receptor sequence was removed from the j, construct using . . .
  

   

  

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