Activity of 3-ketosteroid 9α-hydroxylase (kshab) indicates cholesterol side chain and ring degradation occur simultaneously in mycobacterium tuberculosis*

HIGHLIGHTS

• who: Jenna K. Capyk from the EXPERIMENTAL PROCEDURES Chemicals and Reagents-ADD and, BNC were purchased from Steraloids, Inc(Newport, RI)., AD was purchased from Sigma-Aldrich (St. Louis, MO). Restriction enzymes and the Expand High Fidelity PCR System were purchased from New England Biolabs (Ipswich, MA) and Roche Applied Science, respectively. Oligonucleotides for amplifying kstD were purchased from Integrated DNA Technologies (San Diego, CA) through the Nucleic Acid Protein Service Unit at the University of British Columbia. All other reagents were of HPLC or analytical grade. Water for buffers was purified using a Barnstead Nanopure DiamondTM . . .
  

   

  

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