Adapting to oxygen: 3-hydroxyanthrinilate 3,4-dioxygenase employs loop dynamics to accommodate two substrates with disparate polarities

HIGHLIGHTS

  • who: Yu Yang from the (UNIVERSITY) have published the research work: Adapting to oxygen: 3-Hydroxyanthrinilate 3,4-dioxygenase employs loop dynamics to accommodate two substrates with disparate polarities, in the Journal: (JOURNAL) of May/18,/2018
  • what: Using O2 saturated buffer, the kcat of N27A reached the same level of wtHAO, indicating that the primary reason for a substantial negative impact of the N27A mutation in catalysis with the regular buffer is a decreased oxygen-binding affinity.
  • how: The authors chose 3-hydroxyanthranilate 34-dioxygenase (HAO)3 a ring-cleaving dioxygenase as . . .

     

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