HIGHLIGHTS
- who: Xiaoxi Li from the After obtaining informed consent, human adipose stem cells were isolated from human adipose tissue obtained from healthy donors (22, years old) by liposuction at the Orthopedics, st Hospital of Harbin Medical UniversityIn addition, mouse adipose stem cells were isolated from mouse groin adipose tissue. The fatty tissue was thoroughly cleaned with phosphate-buffered saline (PBS) three times and sliced into small pieces. The tissue sections were digested by adding, .5% type I collagenase (Sigma, USA) at , ℃ and shaking for an hour. The mixture was separated using gradient centrifugation, the supernatant was pelleted . . .
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