Adjuvant formulated virus-like particles expressing native-like forms of the lassa virus envelope surface glycoprotein are immunogenic and induce antibodies with broadly neutralizing activity

HIGHLIGHTS

• who: Helena Müller from the , supplemented with, % FBS and , mM, glutamine, U/ml penicillin and , µg/ml streptomycin, and , × NEAA and sodium pyruvate (Gibco), cells (, ×, well) were differentiated using , nM phorbol, myristate, acetate (PMA, Sigma-Aldrich)73. PMA was removed after , days of treatment. Cells were then grown for an additional , days in supplemented RPMI, . All cell cultures were grown under standard conditions in a humidified , °C, % , incubator. For vectorial expression of arenavirus GPs in eukaryotic cells, we used the mammalian expression vector pCAGGS74. The pCAGGS plasmid encoding the ORF of full-length GPC of LASV . . .
  

   

  

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