HIGHLIGHTS
- What: In this review, the authors aim to give a snapshot of available long-read sequencing technologies, current long-read single-cell isolation protocols, and downstream bioinformatics analysis methods long-read scRNA-Seq data (Fig 1). It is important to consider the specific goals of the experiment and the quality of the tissue available when deciding which approach to use (Sant et_al 2023). snRNA-Seq protocols can be applied to snap-frozen samples, avoiding many of the dissociation-related artifacts (Krishnaswami et_al 2016; Lake et_al 2016).
- Who: Pallawi Kumari from the Institute of Microbial Technology . . .
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