Advantages, pitfalls, and developments of all optical interrogation strategies of microcircuits in vivo

HIGHLIGHTS

SUMMARY

    All-optical approaches leveraging on a combination of multiphoton functional imaging or electrophysiology and single-photon optogenetics for closedloop optical approaches will not be discussed in the context of this review. This approach measures largely subthreshold (synaptic) inputs as the largest amount of molecules are in the neuronal compartment with the largest area/volume ratio, the dendrites (Petersen et_al, 2003; Newton et_al, 2021). Calcium activity is of great interest in neuroscience mostly because its dynamics corresponds to suprathreshold activity (Kerr et_al, 2005; Siegle et_al, 2021), due to the fact that it is one of . . .

     

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