Affinity tag coating enables reliable detection of antigen-specific b cells in immunospot assays

HIGHLIGHTS

  • who: Sebastian Köppert and colleagues from the Institute of Anatomy and Cell Biology, Friedrich-Alexander University Erlangen-Nürnberg, Institute of Neuroanatomy, Medical Faculty, University of Bonn, Bonn, Germany have published the research: Affinity Tag Coating Enables Reliable Detection of Antigen-Specific B Cells in Immunospot Assays, in the Journal: Cells 2021, x of /2021/
  • what: Specifically the authors report an enhancement in assay sensitivity and a reduction in required protein concentrations through the capture of recombinant proteins via their encoded hexahistidine (6XHis) antigen coating enabled detection of SARS-CoV-2 Spike receptor binding . . .

     

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