Akr1p-dependent palmitoylation of yck2p yeast casein kinase 1 is necessary and sufficient for plasma membrane targeting*

HIGHLIGHTS

• who: Praveen Babu from the zymolyase (Seikagaku)) and incubated at , °C for , hSpheroplasts were then washed with , ml of spheroplast wash buffer (1., M sorbitol, mM KPO4, pH, .5, mM NaN3) and resuspended in , ␮l of cold lysis buffer (0., M sorbitol, mM triethanolamine, mM EDTA, mM PMSF) containing, ⫻ PIC. Samples were lysed manually using prechilled pestle tissue grinders (Thomas Scientific) on ice and transferred to pre-chilled microcentrifuge tubes. Tissue grinders were rinsed with , ␮l of cold lysis buffer containing, ⫻ PIC, and washes were pooled with corresponding crude lysates. Unbroken cells were removed by centrifugation at . . .
  

   

  

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