Alanine-scanning mutagenesis of the sixth transmembrane segment of gastric h+,k+-atpase α-subunit*

HIGHLIGHTS

• who: Shinji Asano from the Materials-, cells (human embryonic kidney cell line) were a kind gift from ProfJonathan Lytton (University of Calgary, Calgary, Canada). The pc, vector was obtained from Invitrogen Co. (San Diego, CA). A MutanK kit was from Takara Shuzo Co. (Kyoto, Japan). Pfu DNA polymerase was obtained from Stratagene (La Jolla, CA). Restriction enzymes and other DNA- and RNA-modifying enzymes were from Toyobo (Osaka, Japan), New England BioLabs (Beverly, MA), or Amersham Pharmacia Biotech Inc. (Tokyo, Japan)., Methyl8-(phenylmethoxy)imidazo[1, a] pyridine-3-acetonitrile (SCH28080) was obtained from Schering-Plough Co. (Kenilworth . . .
  

   

  

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