Alox15-launched pufa-phospholipids peroxidation increases the susceptibility of ferroptosis in ischemia-induced myocardial damage

HIGHLIGHTS

• who: Xiao-Hui Ma from the Cell culture and establishment of in vitro ischemia model Rat cardiac myoblast cell line H , cells (American Type Culture Collection, Manassas, Virginia, USA) were cultured in high-glucose DMEM supplemented with, % (v/v) FBS, penicillin G (, U/ml), and streptomycin (, μg/ml), under, % , at , °CTo establish an in vitro ischemia model, H , cells were placed in the Ischemia Modular Incubator Chamber (Billups-Rothenberg Inc, USA), filled with mixed gas (5% , and, %, ). Cells in this chamber were cultured at , °C for , h. Preparation of PUFA-enriched cells PUFA-enriched cells were prepared . . .
  

   

  

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