Amplification of a circular episome carrying an inverted repeat of the dfr1 locus and adjacent autonomously replicating sequence element of saccharomyces cerevisiae(∗)

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• who: Tun Huang from the Lack of suitable amplification markers has hindered the use of the yeast system for investigating the mechanism of gene amplification in a eukaryote with a simple genome and well defined genetic systemRecently, methotrexate has been used to select for Saccharomyces cerevisiae mutants with de novo amplification of the dihydrofolate reductase gene (DFRl) (Huang, T. (1993) In Vivo Disruption and de Novo Amplification of the DFRI Gene Encoding Dihydrofolate Reductase in Saccharomyces cereuisiae, Ph. D. thesis, University of Alberta, Edmonton, Canada). We report here the detailed structure of a DFRI episome amplified in . . .
  

   

  

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