An advanced and ef cient asymmetric pcr method for microarray applications

HIGHLIGHTS

  • who: . and collaborators from the Centro de Investigaciones en Optica, Mexico (IMTEK), University of Freiburg, Freiburg, Germany, Safeguard Biosystems Holding Ltd, London have published the research work: An advanced and ef cient asymmetric PCR method for microarray applications, in the Journal: (JOURNAL)
  • what: The authors demonstrate the principles of this new asymmetric PCR method by amplification and detection of known concentrations of Staphylococcus aureus genomic DNA spiked with human DNA. One of the major reasons for unspecific amplification with excessive cycling is due to the annealing of PCR products 3u2032-OH to the available genomic . . .

     

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