An integrated analytical approach for the characterization of probiotic strains in food supplements

HIGHLIGHTS

SUMMARY

    Compared to classical microbiological methods, flow cytometry and RT-qPCR provide the optimal balance of cost in quality_control screening scenarios. In this work, flow cytometry in combination with RT-qPCR was tested for the first time as a specific quantification and qualification quality_control method for Lactobacillus and Bifidobacterium-based products. In the future, the application of TaqMan probes (Thermofisher Scientific, Waltham, MA, USA) with better specificity will produce faster results in the quality_control workflow. Of 17 These preliminary tests allowed a full verification of all the species declared by the supplier and the certification . . .

     

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