An interaction between the human t cell leukemia virus type 1 basic leucine zipper factor (hbz) and the kix domain of p300/cbp contributes to the down-regulation of tax-dependent viral transcription by hbz*

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• who: Isabelle Clerc from the purified by Ni2⫹-nitrilotriacetic acid-agarose chromatography (Qiagen), as described previously (13)Purified proteins were dialyzed against TM buffer containing , mM Tris, pH, .9, ., mM MgCl2, mM KCl, mM EDTA, pH, .0, mM dithiothreitol, .025% (v/v) Tween, and, % (v/v) glycerol, aliquoted, and stored at ⫺, °C. The dialysis buffer for Tax also included , ␮M ZnSO4. His6-tagged p, and FLAG-tagged CBP were expressed from recombinant baculoviruses (obtained from J. Kadonaga, University of California) in Sf, cells and purified as previously described (45). In vitro translation of HBZ proteins was performed . . .
  

   

  

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