An optimized circular polymerase extension reaction-based method for functional analysis of sars-cov-2

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  • who: GuanQun Liu from the The bacterial artificial chromosome (BAC) encoding a GFP reporter SARS-Co, in the background of hCoV-19/Germany/BY-pBSCoV2-K49/, (GISAID EPI_ISL_2732373) was kindly provided by Armin Ensser (Friedrich-Alexander University Erlangen-Nu00fcrnberg, Germany) and has been described previously [19]pGLCPERlinker was assembled from synthetic DNA oligonucleotides and fragments (IDT) as well as the ampicillin resistance cassette and the origin of replication derived from p, (NEB). have published the Article: An optimized circular polymerase extension reaction-based method for functional analysis of SARS-CoV-2, in the Journal: (JOURNAL)
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