An optimized messenger rna vaccine candidate protects non-human primates from zika virus infection

HIGHLIGHTS

• who: Brooke Bollman from the Generation of modified mRNA and LNPs Modified mRNA was synthesized using in vitro , RNA polymerase-mediated transcription from a linearized DNA template, with, methyl-pseudouridine triphosphate and cap , to improve translation efficiency Formulations of m, and m, were prepared by dissolving ionizable lipids, distearoylphosphatidylcholine, cholesterol, and polyethylene glycol-lipid in ethanol (molar ratios of, :10:38:5:1:5). This lipid mixture was combined with mRNA in a citrate buffer (, mM, pH, .0) at a ratio of, :, (aqueous:ethanol). The formulations were dialyzed against phosphate-buffered saline (pH, .4) for andlt have . . .
  

   

  

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