An optimized pipeline for live imaging whole arabidopsis leaves at cellular resolution

HIGHLIGHTS

• who: Kate Harline from the Plants grown in agar have a natural tendency to shift over time because the roots grow gravitropically and subsequent leaves emerge from the meristemThese developmental events shift the sample in the plate. As early leaf development proceeds, the three dimensionality of leaves becomes more apparent and imaging their entirety becomes more difficult (Fig., ). Early leaf development includes a bend that develops between the petiole and leaf blade in almost all leaves and a variety of curvature and margin patterning differences amongst mutant lines [14-16, ](Figs. F, A, C). This can be . . .
  

   

  

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