An r-based reproducible and user-friendly preprocessing pipeline for cytof data [version 2; peer review:

HIGHLIGHTS

SUMMARY

    Between data generation and downstream data_analysis, data preprocessing is an multi-step procedure required to convert raw FCS files into data objects that can be input to downstream statistical analysis and visualization7. This new version of CATALYST is based on Bioconductor`s SingleCellExperiment class, the standard for high dimensional single cell data_analysis. The cofactor used for transformation is stored inside the object`s internal metadata (int_metadata(sce)$cofactor), and the FCS file of origin for each cell under cell metadata column sample_id (accessible via colData(sce)$sample_id or, equivalently, sce$sample_ id). The authors . . .

     

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