Analyses of a chromosome-scale genome assembly reveal the origin and evolution of cultivated chrysanthemum

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• who: Aiping Song from the Plant materials and genome sequencing We sequenced the entire genome of a previously characterized haploid (n=3x=27) line of potted and ground-type Cmorifolium cv. u2018Zhongshanzigui` (2n=6x=54), derived from the in vitro culture of nonfertilized ovules, (Supplementary Fig., ). Genomic DNA was extracted from young leaf tissue using a DNAsecure Plant Kit (TIANGEN, Beijing, China) for Illumina short read sequencing, PacBio long read sequencing, X Genomics and Hi-C library construction and sequencing, as described in Supplementary Note, . Total RNA was extracted from various organs using an RNA extraction kit . . .
  

   

  

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