HIGHLIGHTS
- who: Вавиловский журнал генетики и and colleagues from the Institute of Сhemical Biology аnd Fundamental Medicine SB RAS, Novosibirsk State University, Novosibirsk, Russia have published the paper: Analysis of domain specificity of the protective chimeric antibody ch14D5a against glycoprotein E of tick-borne encephalitis virus, in the Journal: (JOURNAL)
- what: The aim of this work was to identify the domain of glycoprotein E to which the protective antibody ch14D5a binds.
SUMMARY
Фрагменты ДНК, кодирующие рекомбинантные белки rED3_294, rED3_301, rED1+2 и rE, были получены методом ПЦР по матрице кДНК гена Е вируса клещевого энцефалита, штамм Sofjin-Ru (GenBank: AEP20480.1), с использованием праймеров: rED3_301_NcoI_dir: 5′-GCGCCATGGCCG GCGGTGGCTCGGGTCTTACATACACAATGTGCG-3′; rED3_294_NcoI_dir: 5′-GCGCCATGGCCGGCGGTG GCTCGCTAGAAAAACTTAAGATGAAAGGTC-3′; rED3_his_NotI_rev: 5′- TTAGCGGCCGCTTAGTGATG GTGATGATGATGACTCCCTTTTTGGAACCATTG . . .
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