Androgen-induced exosomal mir-379-5p release determines granulosa cell fate: cellular mechanism involved in polycystic ovaries

HIGHLIGHTS

• who: Reza Salehi from the streptomycin (, u03bcg/ml), penicillin (, U/ml), and FSH (, ng/ml)The culture medium was changed every other day. Follicular diameter was measured daily as the average distance between the outer edges of the basement membrane in two perpendicular planes, and results were expressed as change in follicular volume [9, ]. Exosome release inhibition and miRNA transfection in preantral follicles were carried out by using n=, follicles in each experimental group as described above. Alexa-647-labelled miR-379-5p mimic was used to confirm successful transfection of granulosa cells in preantral follicles. Transfected . . .
  

   

  

  If you want to have access to all the content you need to log in!

  Thanks :)

  Username or E-mail:

  If you don't have an account, you can create one here.