Angiopoietin-like 4 induces a β-catenin-mediated upregulation of id3 in fibroblasts to reduce scar collagen expression

HIGHLIGHTS

• who: Ziqiang Teo from the PLA experiments were performed as described by manufacturer (Olink Biosciences, Sweden) using pairs of antibodies to probe for the protein interactions Rabbit polyclonal , (1:50) was paired with mouse monoclonal anti-c, (1:50) or anti-β-catenin (1:50). The Duolink In Situ Detection Reagents Orange kit was used to reveal the interacting complexes. Sections were counterstained and mounted in Vectorshield mounting media with DAPI (Vector Laboratories, USA). Negative controls did not include the primary antibody. PLA images were taken using the LSM , confocal microscope (Carl Zeiss, Germany) with the Plan-Apochromat . . .
  

   

  

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