HIGHLIGHTS
SUMMARY
Bacterial cultures All manipulations with live Brucellae were performed according to standard operational procedures (SOPs) in a biosafety level 3 (BSL-3) facility at the Swiss Tropical and Public Health Antibiotic persistence of intracellular Brucella abortus Institute in Basel, Switzerland. In cellulo infection model to analyze inhibition of replication at different antibiotic concentrations (MICic) 1.5x104 RAW macrophages per well were seeded onto glass 96-well plates and infected as described above. At 5 hpi the medium was exchanged with DMEM/10% FCS with 10 μg/ml GEN and respective Antibiotic persistence of intracellular . . .
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