Antimicrobial evaluation of sequentially extracted leaf of

HIGHLIGHTS

• who: Teshale Etiso from the Chloroform, % (analytical grade, Trust Chemical Laboratories), hexane, % (analytical grade, Loba Chemie PVT.LTD), ethyl acetate, .8% (analytical grade, Loba Chemie PVT.LTD), methanol, % (analytical grade, Abron Exports-, India) and Mueller Hilton agar media (HiMedia, India) is used as culture media procured from Merry Chemicals Supplier, Addis Ababa. Hydrochloric acid, Wagner`s reagent, sodium hydroxide, powder ferric chloride, acetic anhydride, and sulfuric acid reagents were donated by Jimma University Pharmaceutical Quality Control Laboratory. And Gallic acid standard was obtained from the Jimma University Chemistry department. Plant collection, identification, and preparation have published the . . .
  

   

  

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