Antimicrobial histones and dna traps in invertebrate immunity

HIGHLIGHTS

• who: Aurore C. Poirier from the peptide conjugated to Keyhole limpet hemocyanin (KLH) (Proteogenix)Fixed and permeabilized hemocytes were first incubated for , min in , mM NH Cl and then for , min in PBS containing, % BSA. Then, ␮g/ml antibody dissolved in PBS containing, % BSA was added to coverslips and incubated for , h. After three washes in PBS, ␮g/ml anti-rabbit secondary antibody coupled to Alexa Fluor , was added and incubated for , h. Coverslips were then washed, stained with DAPI, and mounted with fluorescent mounting medium (DAKO). Monitoring of Reactive Oxygen Species Production-Hemocytes freshly withdrawn from . . .
  

   

  

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