Antitumor effects of ral-gtpases downregulation in glioblastoma

HIGHLIGHTS

• who: Tu00e0nia Cemeli et al. from the Cells in D cultures were trypsinized, centrifuged for , min at, u00d7 g, washed with neurobasal media (GIBCO), and resuspended in tumorsphere media (, mL neurobasal media + , mL , supplement + , u00b5L Glutamax + , u00b5L of penicillin/streptomycin + , u00b5g FGF + , u00b5g EGF), and , cells were seeded in ultra-low attachment surface platesFloating cells were transferred , h later into new ultra-low attachment surface plates for tumorspheres growth. When they reach an appropriate size (five cells minimum), tumorspheres were concentrated by gravity in a , mL tube for , min. Then, tumorspheres were washed in PBS with trypan . . .
  

   

  

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