Aplp1 is endoproteolytically cleaved by γ-secretase without previous ectodomain shedding

HIGHLIGHTS

• who: Linda Schauenburg from the , terminally FLAG tagged proteins in the conditioned medium were immunoprecipitated by adding , u00b5l, FLAG antibody (Sigma) and , u00b5l protein G sepharose (GE Healthcare) and incubated overnight in a rotating shaker at , u00b0CSoluble , species in the conditioned medium of untransfected SH-SY Y cells were specifically enriched by overnight incubation with , u00b5l of the polyclonal antibody u03b1APLP1ecto (RandD Systems) and , u00b5l protein G sepharose at , u00b0C. After centrifugation sepharose pellets were washed thrice with buffers with decreasing salt concentration and finally resuspended in gel loading buffer. Samples were subjected to SDS-PAGE and . . .
  

   

  

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