Apolipoprotein e isoform dependently affects tat-mediated hiv-1 ltr transactivation

HIGHLIGHTS

• who: Nabab Khan from the U MG glioblastoma cells were cultured in, × DMEM (Invitrogen) supplemented with, % fetal calf serum and penicillin/streptomycin (Invitrogen)U MG cells were stably transfected with luciferase reporter gene under the control of , LTR promoter following neomycin (Sigma-Aldrich) selection pressure. These cells were provided generously by Dr. Lena Al-Harthi (Rush University, Chicago). Apo, and Apo, were purchased from SigmaAldrich, and Apo, from Abcam. Human plasma HDL were obtained from Kalen Biomedical and ApoE-deprived , (d=1.125-1., g/ml) were prepared [29]. Luciferase reporter assay for Tat-mediated , LTR transactivation . . .
  

   

  

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