Aqp3 is regulated by pparγ and jnk in hepatic stellate cells carrying pnpla3 i148m

HIGHLIGHTS

• who: Matteo Tardelli from the HSCs were isolated from surgical liver resections unsuitable for transplantation, all experimental protocols were approved by the Ethics Committee of Medical University of Florence, after informed consent from all subjects, and carried out in accordance with relevant guidelines and regulationsAfter mechanical digestion, a multi-step enzyme digestion with a collagenase/ pronase/DNAase, solution was performed. Hepatic cells were centrifuged and separated using a density gradient (Percoll, GE Amersham, Arlington Heights, IL). HSCs were seeded on uncoated plastic dishes and cultivated with Iscove's Modified Medium (Dulbecco's medium, EuroClone, Italy) supplemented with . . .
  

   

  

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