Arabidopsis senescence-associated protein dmp1 is involved in membrane remodeling of the er and tonoplast

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• who: Kasaras et_al BMC Plant and colleagues from the S:DMP1-eGFP, mRFP-, [39] and TPK1-mRFP [40] expression vectors were generated and modified as described in [1]RFP-p, [41] and YFP-HDEL were provided by David Robinson (University of Heidelberg, Germany) and Chris Hawes (Oxford Brookes University, UK), respectively. DMP1p:DMP1-eGFP was generated by amplifying a , bp DMP1promoter:, bp , ORF fragment on genomic Arabidopsis Col, DNA with the primers, ′-CGGTCTAGAGAGAACAAAATCCTCCGTATC3′ and, ′-AACTGCAGCGGCAGAGACCGAGGCTTTC3′, followed by digestion of the PCR product with XbaI/ PstI and ligation into XbaI/PstI digested binary vector pGTkan, [1]. Plant material . . .
  

   

  

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