Array-based sequencing of filaggrin gene for comprehensive detection of disease-associated variants

HIGHLIGHTS

  • who: X.F. Colin C. Wong BSc from the (UNIVERSITY) have published the Article: Array-based sequencing of filaggrin gene for comprehensive detection of disease-associated variants, in the Journal: (JOURNAL)

SUMMARY

    The authors designed and optimized overlapping FLG-specific primer assays (containing NGS adapters) to span the entire FLG coding region including known intragenic copy number variation (CNV) (see Fig 1, A; see Table E1 in this article's Online Repository at www.jacionline.org). A total of 48 overlapping primer assays, with amplicons of maximum 500 base_pairs, provided redundancy for sequencing . . .

     

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