Arrdc5 expression is conserved in mammalian testes and required for normal sperm morphogenesis

HIGHLIGHTS

• who: Mariana I. Giassetti from the Single-cell RNA-sequencing and gene ontology analysis Testes from prepubertal Holstein breed cattle and mixed breed pigs were collected by castrationFor all samples, testicular single-cell suspensions were generated using the methodology previously described by refs., . Briefly, testicular parenchyma was enzymatically digested with trypsin-EDTA solution (Sigma-Aldrich) followed by Collagenase IV (Worthington) with DNase I (Sigma-Aldrich) using gentleMACS Octo-Dissociator (MiltenyiBiotech). Enzymatic activity was then blocked (10% FBS have published the Article: ARRDC5 expression is conserved in mammalian testes and required for normal sperm morphogenesis, in the Journal . . .
  

   

  

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