HIGHLIGHTS
- who: David Rozema from the (UNIVERSITY) have published the paper: Artificial Chaperone-assisted Refolding of Carbonic Anhydrase B, in the Journal: (JOURNAL)
- what: The authors provide a detailed study of carbonic anhydrase B (CAB) refolding assisted by these "artificial chaperones." The studies show that the detergent can capture the unfolded protein even at submicellar concentrations, but that not all CAB-detergent complexes lead efficiently to refolded enzyme upon introduction of the stripping agent. The authors will use the term "dilution additive technique" to describe the general approach pioneered by Horowitz and co-workers . Most of . . .
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