Asc-mediated nf-κb activation leading to interleukin-8 production requires caspase-8 and is inhibited by clarp*

HIGHLIGHTS

• who: Mizuho Hasegawa from the Reagents-MDP was purchased from SigmaRecombinant TNF-␣ was purchased from Genzyme (Cambridge, MA). Recombinant soluble mouse Fas ligand was prepared as previously described (19)., VADfmk, IETD-fmk, and, AAD-CMK were purchased from Calbiochem (La Jolla, CA). Anti-p, mAb (clone, ) was purchased from BD Transduction (Lexington, KY). Anti-FLAG (M2) and anti-HA mAb (HA-7) were purchased from Sigma. Anti-human caspase, and anti-glyceraldehyde-3-phosphate dehydrogenase mAb were purchased from MBL (Nagoya, Japan) and Chemicon (Temecula CA), respectively. Anti-ASC mAb was kindly provided by Dr. Junji Sagara . . .
  

   

  

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