Assay-ready cryopreserved cell monolayers enabled by macromolecular cryoprotectants

HIGHLIGHTS

SUMMARY

    This polyampholyte enhanced the post-thaw recovery of a panel of cell lines (A549, MC-3T3, and Neuro-2a cells) compared to DMSO alone and the suspension cryopreservation of stem_cells. High cell recovery was demonstrated for adherent cell lines that are widely used in the drug discovery field including A549, HepG2, and Caco-2 cells. Banked "assay-ready" cells were frozen at multiple cell densities, designed for adaptability toward desired applications, and could be stored for over 1 month in a conventional -80 °C freezer. Exploring different cell lines (e_g, to look for a . . .

     

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