Assessment of endothelial colony forming cells delivery routes in a murine model of critical limb threatening ischemia using an optimized cell tracking approach

HIGHLIGHTS

  • who: Marta Rojas-Torres from the University, Cu00e1diz, Spain have published the research work: Assessment of endothelial colony forming cells delivery routes in a murine model of critical limb threatening ischemia using an optimized cell tracking approach, in the Journal: (JOURNAL)
  • what: Herein, we describe an optimized workflow for an accurate in_vivo and ex vivo cell tracking analysis with DiR, a NIR lipophilic labeling dye, for its use in preclinical biodistribution assays.
  • how: Optimization of DiR labeling Determination of the relation between DiR intensities and cell numbers First a standard curve was designed . . .

     

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