Automated multimodal fluorescence microscopy for hyperplex spatial-proteomics: coupling microfluidic-based immunofluorescence to high resolution, high sensitivity, three-dimensional analysis of histological slides

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  • who: Laura Furia and colleagues from the United States University of Palermo, Italy have published the article: Automated multimodal fluorescence microscopy for hyperplex spatial-proteomics: Coupling microfluidic-based immunofluorescence to high resolution, high sensitivity, three-dimensional analysis of histological slides, in the Journal: (JOURNAL)
  • what: In the experiments performed to compare automated and manual staining efficiency an Alexa Fluor 790 secondary antibody was employed to minimize background thanks to the complete absence of autofluorescence in this spectral range. In all the analysis the mean intensity per pixel per cell was employed as a reference for . . .

     

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